共表达OPG与bFGF的复制缺陷型腺病毒修复牙槽骨缺损的实验研究

The experimental study of the restoration of alveolar bone defects by replication deficient recombinant adenovirus encoding osteoprotegerin and basic fibroblast growth factor

目的探讨以骨保护素(OPG)和碱性成纤维细胞生长因子(bFGF)为外源基因,构建双表达重组腺病毒载体,结合胶原膜促进牙槽骨骨缺损修复,为牙周病治疗提供新方法和新思路。方法在6只犬下颌两侧的第3、4前磨牙根分叉造牙槽骨缺损,每只犬的一侧为实验组,另一侧为材料对照组或空白对照组。实验组:翻瓣造牙槽骨缺损,置胶原膜并滴加重组腺病毒,缝合龈瓣,局部注射腺病毒;材料对照组:翻瓣造牙槽骨缺损,置胶原膜,缝合龈瓣;空白对照组:翻瓣造牙槽骨缺损,直接缝合。手术后通过影像学和组织学方法观察骨缺损的再生情况。结果术后6周,实验组骨缺损区有新骨形成,新生骨充满缺损区;材料对照组骨缺损区有新骨形成,新生骨钙化程度较低;空白对照组骨缺损区基本没有新生骨形成,骨小梁稀疏,主要为疏松结缔组织。术后12周,实验组骨缺损区新生骨量明显增加,新生牙槽骨已充满根分叉区,骨小梁致密;材料对照组骨缺损区新生骨量明显增加,但未达到根分叉顶,骨缺损区有大量新生牙槽骨但未充满根分叉区;空白对照组结果与术后6周类似。结论以OPG和bFGF为外源基因的双表达重组腺病毒可促进犬牙槽骨缺损的修复。

Objective To develop a new method to treat periodontitis by using tissue and gene engineering technology, and explore the restoration of alveolar bone defects with recombinant adenovirus encoding osteoprotegerin （OPG） and basic fibroblast growth factor（bFGF）. Methods Class HI furcation defects were created in the mandibular premolars. The collagen membrane with adenovirus was covered on the defects in the experimental group and membrane without adenovirus inthe control group, while nothing in the other group. Six and twelve weeks after surgery,X-ray and histologic examination were carried out. Results The new bone filled around almost the entire circumference of the furcation in the experimental group, the quantity of the new bone was less in the control group, while defects filled with connective tissue. Conclusion Coexpressing OPG and bFGF could promote alveolar bone regeneration.