DNA IQ磁珠法结合Maxwell^(TM) 16自动仪提取接触DNA

Extraction of DNA from "touch" DNA samples by DNA IQ Casework Sample Kit in Maxwell^(TM) 16

目的研究DNA IQ磁珠法结合MaxwellTM 16自动仪对接触DNA提取的应用价值。方法 151份案件接触DNA检材95℃裂解后,采用DNA IQ磁珠法结合MaxwellTM 16自动仪提取DNA,然后进行DNA定量和STR分型检测,统计各种类型的接触DNA含量I、PC CT值和STR分型成功率。结果 151份案件接触DNA检材中,除果核平均DNA获得量为9.51ng以外,其它接触检材的平均DNA获得量均大于10ng,烟蒂检验成功率最高为93%,果核检验成功率较低,为60%。所有DNA样品的IPC CT值均在27左右,纯度高。结论大部分接触DNA检材采用DNA IQ磁珠法结合MaxwellTM 16自动仪可提取到足以进行STR分型的DNA。

Objective To study the application of DNA IQ Casework Sample Kit in MaxwellTM 16 Instrument for ＂touch＂ DNA extraction.Methods After 95℃ direct lysis protocol,151 ＂touch＂ DNA samples were extracted in MaxwellTM 16 by DNA IQ Casework Sample Kit.The DNA concentration,IPC CT and success rate of STR typing were observed between PCR quantification and STR analysis.Results In 151 ＂touch＂ DNA samples,the average DNA yields were more than 10ng,except the DNA from fruit-stones,which average yield was 9.51ng.The success rate of STR typing from cigarette butts is 93%,and the success rate of STR typing from fruit-stones is 60%.All DNA IPC CT was about 27.Conclusion The most of ＂touch＂ DNA samples could get enough DNA for STR typing by DNA IQ Casework Sample Kit in MaxwellTM 16 Instrument.