原子力显微术中的生物样品自组装方法

Biological Sample Preparation via Self-assembly Methods for Atomic Force Microscopy Studies

原子力显微成像技术作为研究蛋白质、核酸、细胞等生物样品的有效手段,已被广泛应用于生命科学的各个领域。原子力显微成像前,在基底上成功地制备生物样品自组装层是其研究能否顺利进行的首要前提。本文系统地综述了原子力显微镜(AFM)基底的选择以及蛋白质、脂质膜、DNA、细胞等生物样品成像前常用的自组装方法,着重阐述样品与基底的固定方式,并展望了其发展趋势。

As an effective tool to study proteins,nucleic acids,cells and other biological samples,atomic force microscope（AFM） has been widely used in various fields of life science.Biological sample preparation via self-assembly is the precondition of the research.In this paper,common self-assembly methods of biological samples such as proteins,lipid films,DNA and cells for AFM studies are reviewed,and how the samples to be well attached to a substrate using appropriate,nondestructive methods is discussed.The trends towards the development in the future are also looked into.