慢性阻塞性肺疾病患者尿白三烯E4的变化

The change of urinary leukotriene E4 in patients with chronic obstructive pulmonary disease

目的探讨慢性阻塞性肺疾病(COPD)患者尿白三烯E4(Ieukotriene E4 LTE4)的变化。方法采用竞争性酶联免疫吸附试验技术(ELISA)测定15位COPD患者急性加重期的尿LTE4水平,并与8位健康对照组者比较;同时对COPD患者的尿LTE4与其第一秒用力呼气容积(FEV1)、FEV1/用力肺活量(FVC)、外周血中性粒细胞(pNC)及外周血嗜酸性粒细胞计数(pEC)进行相关性分析。结果 COPD患者急性加重期尿LTE4明显高于健康对照组(P<0.01)。尿LTE4值和FEV1呈显著负相关(r=-0.607,P<0.01,n=23),和FEV1/FVC呈显著负相关(r=-0.697,P<0.01,n=23),和pNC无显著相关性(r=0.218,P>0.05),和pEC无显著相关性(r=-0.032,P>0.05)。结论 COPD患者急性加重期尿中LTE4增高。提示LTE4可能与COPD的发病机制有关。

Objective To evaluate the change of urinary leukotrience E4（LTE4） in patients with chronic obstructive pulmonary disease.Methods 15 patients with COPD were tested the urine concentration of LTE4 by ELISA in acute exacerbation,and compared to 8 healthy controls.In addition,the correlation between the urine concentration of LTE4 and forced expiratory volume in one second and forced expiratory volume in one second/forced vital capacity,periperal neutrophils,periperal eosinophil count of patients were investigated.Results Urinary LTE4 in exacerbations period was significantly higher than control group（857.85±393.05 vs 239.00±24.24 pg/ml,P〈0.01）.There was a significant negative relation between urinary LTE4 and forced expiratary volume in one second（r=-0.607,P〈0.01,n=23）.And a significant negative relation between urinary LTE4 and forced expiratary volume in one second/forced vital capacity（r=-0.697,P〈0.01,n=23）.There was no correlation between urinary LTE4 and periperal neutrophils count（r=0.218,P〉0.05）,and periperal eosinophil count（r=-0.365,P〉0.05）.Conclusion Urinary LTE4 in patients with COPD was significantly higher than control group,which suggested that LTE4 participated in the pathogenesy.