义眼台植入对眶骨组织中转化生长因子－β1表达的影响

Expression of TGF-β1 protein in orbital bone after enucleation with placement of an orbital implant

【摘要】背景义眼台植入是防治眼球摘除术后眼眶畸形发育的主要手段，但其作用机制尚不十分清楚。研究表明骨局部生长因子在骨组织的发生、发育、损伤修复等过程中具有重要作用，转化生长因子-β1（TGF-β1）的作用更值得关注。目的检测眼球摘除和义眼台植入后眶骨组织中骨局部TGF-β1．的表达情况，探讨眼球摘除后眼眶畸形发育防治的作用机制。方法选用同龄、健康、体质量相近的新西兰幼兔21只，按随机数字表法分为眼球摘除组、义眼台植入组和正常对照组。实验兔1月龄时对眼球摘除组和义眼台植入组分别行左眼球摘除或眼球摘除联合义跟台植入术，正常对照组左眼眶作为对照。术后1个月时处死全部兔，用酶免疫组织化学法和FITC免疫荧光标记法对眼球摘除组、义眼台植入组和正常对照组兔眶骨组织中TGF-β1蛋白表达情况进行检查，利用ELISA法测定兔眶骨组织中TGF—p，蛋白的表达水平。结果术后1个月义眼台植入组和正常对照组兔眶高和眶宽的数值均明显高于眼球摘除组，差异均有统计学意义（眶高：P=0．00、P=0．00；眶宽：P=0．00、P=0．00）。免疫组织化学检测发现，义眼台植入组和正常对照组兔眶骨组织中TGF-β1，的染色强度明显高于眼球摘除组，骨组织中着色细胞多，染色较深，而义眼台植入组和正常对照组兔眶骨组织中TGF-β1的染色强度相近。TGF-β1免疫荧光法染色结果表明，义眼台植入组和正常对照组兔眶骨组织中TGF--β1的荧光细胞数量多于眼球摘除组。ELISA检测结果显示，眼球摘除组兔骨组织中TGF-β1的质量分数较义眼台植入组和正常对照组低，差异均有统计学意义（P=0．00、P=0．00），义眼台植入组和正常对照组骨组织中TGF-β1，质量分数的差异无统计学意义（P=0．41）。结论TGF-β1参与了眶骨发育过程及义眼台植入防治眼球摘除后眼眶畸形发育的过程。

Background Placement of an orbital implant is a main way to prevent orbital atrophy with aging. But its mechanism is under clear. Researchs showed that bone growth factors play important role during the development and repair of bone,especially transforming growth factor-β1（TGF-β1 ）. Objective Present study was to investigate the expression of TGF-β1 protein in orbital bone after enueieation or enucieation with placement of an orbital implant and its function in the mechanisms of preventing and treating the orbital malformed development after enucleation with placement of an orbital implant. Methods Twenty-one age- and weight-matched New Zealand white young rabbits were randomly divided into the enucleation,implant and control groups,and each group including seven rabbits. Eyeball nucleation surgery was performed in the left eyes of 7 1-month-old rabbits, and a spherical orbital implant was inserted after enucleation of the left in matched rabbits in implant group. The left eye of normalrabbits served as controls. Tbe rabbits were sacrificed in 1 month after surgery. The expression of TGF-β1 protein in the left orbital bone was detected using enzyme immunoassay and FITC labelling immunoassay technique in the sections of zygomatic bones. The content of TGF-β1 protein in the left orbital bone tissue was measured by ELISA method. This use of animals complied with the Regulations for the Administration of Affair Concerning Experimental Animals by State Science and Technology Commission. Results The height and width of orbital in enucleation group were significantly lower than those of implant and normal control groups （ height ： P = 0.00, P -- 0.00 ; width ： P = 0.00,P= 0.00）. The positive bone ceils of both enzyme immunoassay and FITC staining were increased in the implant and control groups in comparison with enucleation group, but the positive response intensity for TGF-β1 was resembled between implant group and control group. ELISA result revealed that the content of TGF-β1protein in bone tissue was significantly lower in the enucleation group than in implant and control groups （ P = 0.00, P = O. 00 ）. The expression and content of TGF-I31 protein in bone tissue is similar between the implant group and the control group（ P =0. 41）. Conclusion The experiment results indicate that TGF-β1 protein participate in the orbital development. TGF-β1 played important role in the prevention and treatment of enucleation-induced orbital malformation in the eye with placement of an orbital implant.