抗精神分裂症药物诱发迟发性运动障碍与COMT基因多态性的关联性分析

Analysis on association between COMT gene polymorphism and tardive dyskinesia induced by antipsychotic drugs

目的:通过分析中国北方汉族人群中精神分裂症患者COMT基因多态性Val158Met分布,研究COMT基因多态性与精神分裂症及迟发性运动障碍(TD)发生的关系。方法:采集356例并发TD的精神分裂症患者(TD组)、419例不发生TD的精神分裂症患者(非TD组)及471例正常健康人(正常对照组)的全血样本,提取基因组DNA,应用TaqMan探针检测COMT基因多态性Val158Met基因型和等位基因的分布。结果:正常对照组与精神分裂症组比较,基因型与等位基因频数分布差异均无统计学意义(χ2=3.08,df=3,P=0.21;χ2=2.067,df=2,P=0.15)。TD组与正常对照组比较,基因型与等位基因频数分布差异均无统计学意义(χ2=1.857,df=3,P=0.40;χ2=1.281,df=2,P=0.26)。非TD组与正常对照组比较,基因型与等位基因频数分布差异均无统计学意义(χ2=2.505,df=3,P=0.29;χ2=1.709,df=2,P=0.19)。TD组与非TD组比较,基因型与等位基因频数分布差异均无统计学意义(χ2=0.021,df=3,P=0.99;χ2=0.013,df=2,P=0.91)。结论:精神分裂症的发生与COMT基因多态性Val158Met无关联性,精神分裂症患者并发TD与COMT基因多态性Val158Met无关联性。

Objective To observe the distribution of COMT gene polymorphism Val158Met in Chinese patients with schizophrenia and investigate the possible association between COMT gene polymorphism and schizophrenia and tardive dyskinesia（TD）.Methods The whole blood samples from 356 schizophrenic patients with TD（TD group）,419 schizophrenic patients without TD（non-TD group）and 471 cases of normal healthy subjects（control group） were collected,the DNA was extracted,the distribution of genotype and allele of COMT gene polymorphism Val158Met was detected by Taqman assays.Results Compared between schizophrenic patients group and normal control group,the allelic frequency and genotypic frequency of rs4680 hadn＇t significant differences（χ2=3.08,df=3,P=0.21;χ2=2.067,df=2,P=0.15）.Compared between TD group and normal control group,the allelic frequency and genotypic frequency of rs4680 hadn＇t significant differences（χ2=1.857,df=3,P=0.40;χ2 =1.281,df=2,P=0.26）.Compared between non-TD group and normal control group,the allelic frequency and genotypic frequency of rs4680 hadn＇t significant differences（χ2=2.505,df=3,P=0.29;χ2=1.709,df=2,P=0.19）.Compared with TD group and non-TD group,the frequencies of allele and genotype of rs4680 hadn＇t significant differences（χ2=0.021,df=3,P=0.99;χ2=0.013,df=2,P=0.91）.Conclusion The Val158Met polymorphism of COMT gene isn＇t associated with schizophrenia and TD.