摘要
用不同强度的静磁场作用于超氧化物岐化酶（ＳＯＤ）溶液后，通过测定其活性表明，ＳＯＤ的活力随磁感应强度和作用时间不同而波动性升高并最终趋于稳定． ２００ ｍ Ｔ磁场作用２ ｈ，ＳＯＤ对自氧化的抑制率（活力）增加１７ ％ ． 实验还首次发现ＳＯＤ的磁效应具有一定的可逆性． 将邻苯三酚自氧化反应测定ＳＯＤ活力的反应体系置于磁场中，则发现ＳＯＤ对自氧的抑制率随磁感应强度不同而波动性降低，随温度升高则降低程度迅速加剧． 在Ｔｒｉｓ介质中降低４４．６ ％ ，在ＰＢＳ中则可完全失活．
The activity of superoxide dismutase(SOD) has been determined after exposing the enzyme solution to magnetic field(MF) with various intensity,the activity increases fluctuately with the ehangl of magnetization time and field intensity,and goes to steady in the end.Exposing the engyme solution to 200 mT MF for 2 hours the inhibition ratio of SOD inereases about 17 %,and the magnetic effect is reversible.The inhibition ratio of SOD decreases fluctuately with the change of field intensity and decreases accelerately at higher temperature.When exposing the system of the pyrogallol autoxidation to magnetic field,the activity of SOD decreases 44.6 % under the condition of 25 ℃ and 460 mT MF.
出处
《湖南大学学报（自然科学版）》
EI
CAS
CSCD
1999年第5期18-23,共6页
Journal of Hunan University:Natural Sciences
基金
国家自然科学基金
关键词
静磁场
超氧化物歧化酶
SOD
可逆性
磁效应
static magnetic field
superoxide dismutase(SOD)
reversible
pyrogallol autoxidation method
