摘要
２０～３０ｍ ｍ ｏｌ／ Ｌ 半胱氨酸在 ２ｍ ｉｎ 内可与 φ＝ ０．０３ 甲醛完全反应而被掩蔽。利用谷胱甘肽与 ５，５′二硫代双（２硝基苯甲酸）（ Ｄ Ｔ Ｎ Ｂ）的颜色反应，测定产物在波长 ４１２ｎｍ 的 Ｏ Ｄ 值，通过计算或标准曲线可迅速得出谷胱甘肽含量，误差小于 ２％ 。
A new method for rapid determination of reduced glutathione in the presence of cysteine was described: 20～30 mmol/L cysteine can be removed after reacting with φ =0.03 formaldehyde in 2 min; By means of color reaction with DTNB, glutathione concentration can be obtained easily with the value of OD 412 of the product. Error is less than 2%.
出处
《华东理工大学学报（自然科学版）》
CAS
CSCD
北大核心
1999年第5期474-476,共3页
Journal of East China University of Science and Technology
