摘要
目的检测livin和半胱氨酸天冬氨酸特异性蛋白酶-3(caspase-3)的mRNA在胃癌组织中的表达,探讨其与胃癌临床病理特征之间的关系。方法采用逆转录-聚合酶链反应检测60例胃癌患者胃癌组织中livin、caspase-3的mRNA表达。结果 Livin mRNA在胃癌无淋巴结转移胃癌的Livin mRNA阳性表达率明显低于有淋巴结转移者(P<0.05),caspase-3 mRNA则相反(P<0.05);Livin在I/Ⅱ期胃癌阳性表达率明显高于Ⅲ/Ⅳ期者(P<0.05),caspase-3则逐渐降低(P<0.05)。Livin阳性表达率随浸润深度增加而逐渐增高caspase-3无差异(P>0.05)。结论 Livin在胃癌的mRNA表达与肿瘤浸润深度、TNM分期及淋巴结转移有关;caspase-3在胃癌的mRNA表达与肿瘤浸润深度、淋巴结转移有关。
Objective To examine the expression livin and caspase-3 mRNA in gastric carcinoma and to determine the relationship of clinical pathologic characteristics.Methods sixty gastric carcinoma tissues were studied for the expression of livin and caspase-3 mRNA by means of reverse transcription polymerase chain reaction（RT-PCR）.Results Livin mRNA expression in gastric cancer patients without lymph node metastasis were significantly higher than those with lymph node metastasis（P〈0.05）,caspase-3 mRNA was lower（P〈0.05）.The positive expression rate of livin in the I/Ⅱ stage was significantly higher than that in the Ⅲ/Ⅳ stage（P〈0.05）,caspase-3 was gradually decreased.The positive expression rate of Livin increased with depth of invasion decreased,caspase-3 was no difference（P〉0.05）.Conclusion The expression of livin was related to the invasion,TNM stage,lymph node metastasis in gastric cancer,while the caspase-3 expression was only related to TNM stage and lymph node metastasis.
出处
《中国医药指南》
2011年第18期179-181,共3页
Guide of China Medicine
