D4020大孔树脂分离姜油树脂中的姜酚

Separation of gingerols from ginger oleoresin with D4020 macroporous resin

为了获得一种高效、洁净的姜酚分离方法,选用乙醇-水体系为柱层析方法流动相,固定相为D4020大孔吸附树脂,从超临界二氧化碳提取的姜油树脂中分离姜酚。用紫外分光光度法对各段洗脱液进行跟踪检测,确定含姜酚的洗出段。用高效液相色谱法(high performance liquid chromatography,HPLC)和气相色谱质谱联用法(gas chromatography-mass spectrometer,GC-MS)对含姜酚的洗脱液进行检测。研究结果表明,在保证86.11%收率的条件下,姜酚的峰面积百分数为71.98%;经二次柱层析纯化后,姜酚的峰色谱面积百分数可达96.27%,收率为76.75%。该方法在分离纯度和收率上都获得了好的效果。

In order to find an efficient clean method to separate the gingerol,a ethanol-water solution was used as mobile phase,and the D4020 macroporous resin was used as the stationary phase.Based on column chromatography analysis,the gingerols were separated from supercritical carbon dioxide extracted ginger oleoresin.Eluates in every stage were measured by UV to find out the sections containing gingerols.Then,the samples containing gingerols were measured by HPLC and GC-MS.The results showed that a average peak area percentage was 71.98% at the yield of 86.11%.After further purification,the peak area percentage of gingerols rose to 96.27% at a yield of 76.75%.This method could achieve good results in both purity and yield.