β-连环蛋白和APC蛋白在胃腺瘤和胃癌中的表达及意义

Expression and significance of β-catenin and APC in gastric adenoma and gastric cancer

目的观察β-连环蛋白和家族性腺瘤样息肉病蛋白(adenomatous polyposis coli protein,APC蛋白)在正常胃黏膜、胃腺瘤和胃癌组织中的表达,探讨其在胃癌发生、发展中的作用。方法经胃镜活检病理检查证实的126例不同胃组织标本,包括40例正常胃黏膜、38例胃腺瘤性息肉(胃腺瘤)和48例胃腺癌组织,以免疫组化ABC法检测标本中中β-连环蛋白和APC蛋白的表达。结果正常胃黏膜、胃腺瘤、胃癌组织β-连环蛋白和APC蛋白异常表达率呈递增趋势,经两两比较,差异均有统计学意义(P<0.05)。胃癌组织中β-连环蛋白和APC蛋白具有显著相关性(r=1.0,P<0.05),胃腺瘤中两者无明显相关性(r=0.169,P=0.430)。结论β-连环蛋白和APC蛋白在胃癌的发生、发展中起重要作用,但两者在胃腺瘤中的相互作用及具体机制有待进一步研究。

Objective To observe the expression of β-catenin and APC in normal gastric mucosa,gastric adenoma and gastric cancer tissues,so as to investigate their significance in the carcinogenesis and progression of gastric cancer.Methods One hundred and twenty-six gastric epithelial biopsy specimens diagnosed by histopathology were obtained,including 40 normal gastric mucosa,38 gastric adenomatous polyps（22 high-grade and 16 low-grade intraepithelial neoplasia） and 48 gastric cancer.Expression of β-catenin and APC were detected by avidin-biotin complex（ABC） immunohistochemical method.Results The abnormal expression rates of β-catenin and APC increased progressively from the normal control group to gastric cancer.The difference was statistically significant（P0.05）.The correlation of β-catenin and APC in gastric cancer was significant,but it was uncorrelated in gastric adenomas.Conclusion β-catenin and APC play important roles in the carcinogenesis and progression of gastric cancer.The correlation of β-catenin and APC in gastric adenomas is pending and need further research.