大鼠颅脑爆震伤模型的建立

Establishment of brain explosive injury model in rats

目的建立稳定、可靠的大鼠颅脑爆震伤模型。方法 SD大鼠96只随机分为8、10、12cm组和对照组(n=24),将大鼠麻醉后分别放置距电雷管(600mgTNT当量)垂直距离8 cm、10 cm、12 cm处进行爆炸,于伤后24h行头颅MRI检查,6h、24h、3d、7d行检测损伤区光、电镜下病理变化。结果 8 cm组大鼠几乎在当天全部死亡,10 cm组大鼠存活时间也较短,少有存活7天,12 cm组大鼠存活时间在7天以上,且伤后24小时MRI提示挫伤区存在血肿及挫裂伤,病理学提示皮质、脑膜、脑室周围、神经元胞体、线粒体等均出现不同程度水肿。结论 600mgTNT电雷管在距离大鼠头部垂直12 cm处可制作稳定的大鼠颅脑爆震伤模型。

Objective To establish a rat brain explosive injury model with stability and reliability.Methods A total of 96 SD rats were randomly divided into sham operated group（n=24） and brain explosive injury group induced by 600mgTNT electric detonator from 8cm、10cm、12cm vertical dimension respectively（n=72）.The brain explosive injury group was subdivided into 6h、24h、3d、7d groups with 5 rats in each.Pathomorphological changes were detected by light microscopy and electron microscopy and the brain hemorrhages were observed by MRI 24 hours after injury.Results 8cm group of rats were almost dead after injuried,10cm group of rats lived longer but no more than 7 days,12cm group of rats all lived more than 7 days.The rats MRI display hemorrhage and contusion,Pathomorphological differences were edema with cortex,meninges,periventricular,neuron body,mitochondria at 24 hours after injury.Conclusion The rat brain explosive injury model with stability and reliability induced by 600mg TNT electric detonator and 12cm distance.