HPLC法测定箭羽糖康片中山柰酚的含量

Content determination of kaempferol in Jianyutangkang tablet by HPLC

目的：建立测定箭羽糖康片中山柰酚含量的方法。方法：采用HPLC法,色谱柱Diamonsil-C18柱（250 mm×4.6 mm,5μm）;流动相为乙腈-0.1%甲酸（33：67）,流速0.8 mL.min-1,检测波长258 nm,柱温45℃。结果：山柰酚色谱峰与其他色谱峰分离良好,阴性样品无干扰。山柰酚在2.076~20.76μg.mL-1范围内呈良好线性关系（r=0.999 8）,样品平均回收率为100.39%,RSD为0.52%。结论：本法简便、准确、重复性好,可用于箭羽糖康片中山柰酚的含量测定。

Objective： To develop the method for quality control of kaempferol in Jianyutangkang tablet.Methods： Diamonsil-C18 column（250 mm × 4.6 mm,5 μm）was used for separation.The mobile phase consisted of acetonitrile-0.1% formic acid（33：67） with the flow rate of 0.8 mL·min-1,and the detection wavelength was set at 258 nm.Results： The kaempferol peak was separated from other peaks without interference by negative sample.The linear range of kaempferol was 2.076-20.76 μg·mL-1（r = 0.999 8）.The recovery was 100.39% with RSD of 0.52%.Conclusion：The method is simple,accurate and repeatable,and it is suitable for the content determination of kaempferol in Jianyutangkang tablet.