摘要
目的建立乳胶增强免疫比浊测定血清胱抑素C(Cystatin C)的全自动分析方法。方法采用乳胶增强免疫比浊法测定Cystatin C,根据美国临床实验室标准化协会相关文件,对方法进行精密度、线性、准确性等评价及临床初步应用。结果此方法批内CV<4.0%,批间CV<5.0%。抗干扰性强,血红蛋白小于或等于4.0g/L、胆红素小于或等于420μmol/L、类风湿因子小于或等于2 200 U/L、三酰甘油小于或等于10 mmol/L对测定无影响;与进口试剂相比,Y=1.007 5X+0.002 9,r=0.999 5,二者相关性良好。试剂开瓶稳定性良好。结论乳胶增强免疫比浊法测定血清Cystatin C,具有方法简便、快速、灵敏的优点,且结果准确,可用自动分析仪测试,适合临床检验应用。
Objective To establish a new automatic method of latex-enhanced immunoturbidimetry for the detection of serum cystatin C. Methods According to relevant documents of NCCLS,the precision,linearity and accuracy of this method were evaluated by latex-enhanced immunoturbidimetry. Results The intra and inter coefficient var iations（CV） of this method were less than 4.0% and 5.0K respectively,and the performance of anti-interference was strong, when the level of hemoglobin was above 4.0 g/L,the level of bilirubin was≤420μmol/L,the level of rheu- matoid factor was≤2 200 U/L, the level of triglyceride was≤10 mmol/L,there was no influence of the test result. A comparison between imported cystatin C（Y）and this kit（X） showed the following results： Y=1. 007 5X＋ O. 002 9,r=0. 999 5. There was a good correlation between the two regents. According to the statistical data, the regents would be stable after first use. Conclusion The detection of serum cystatin C by latex-enhanced immunoturbidimetry is convenient, fast ,accurate and suitable for automation analysis, which can be applied to the clinical implication.
出处
《检验医学与临床》
CAS
2011年第10期1159-1160,1163,共3页
Laboratory Medicine and Clinic
