先兆子痫患者血浆组织因子及其抑制物联合检测的初步应用

The application of combined detection of plasma tissue factor and its inhibitor in patients with pre-eclampsia

目的探讨先兆子痫(PE)患者血浆组织因子(TF)、组织因子抑制物(TFPI)和TF/游离TFPI(f-TF-PI)的变化及其临床意义。方法采用双抗体夹心酶联免疫吸附试验(ELISA)法检测PE患者、正常妊娠晚期孕妇和未怀孕正常体检妇女TFf、-TFPI水平。结果 (1)PE患者与正常妊娠孕妇相比,血浆TF水平明显升高,差异有统计学意义(P<0.01);PE和正常妊娠孕妇与未妊娠妇女相比,血浆TF也明显升高,差异有统计学意义(P<0.01)。(2)PE患者血浆f-TFPI水平明显高于正常妊娠孕妇和未妊娠妇女,差异有统计学意义(P<0.01),但正常妊娠孕妇与未妊娠妇女f-TFPI差异无统计学意义(P<0.05)。(3)PE患者与正常妊娠孕妇和未妊娠妇女相比,TF/f-TFPI显著增加,差异有统计学意义(P<0.05)。结论血浆TF和TFPI与妊娠胎盘形成密切相关,TF、TF-PI及其比值可作为妊娠并发PE的新指标,对于临床上早期诊断和防治PE有重要意义。

Objective To clarify the changes and clinical application of plasma tissue factor（TF） and tissue factor pathway inhibitor（TFPI） in patients with pre-eclampsia（PE）. Methods We used the enzyme linked immunosorbent assay（ELISA） to test the plasma levels of TF and free TFPI in 31 PE, 35 healthy pregnant women,and 36 nonpregnant women. Results （1） The increase of TF levels in women with PE was higher than that of healthy pregnant women,with significant difference（ P〈O. 01）, and the increase of TF levels of healthy pregnant women was higher than that of nonpregnant women, with significant difference（P〈0.01）. （2） There was a significant increase in circulating f-TFPI levels in women with PE（12. 2 ±2. 9 ng/mL）compared with healthy controls（P〈0.01）. But f-TFPI did not singnificantly inrease between healthy pregnant woraen and healthy controls（P〉0.05）. （3）There was a significant increase of TF/f-TFPI ratio in women with PE compared with healthy pregnant women and nonpregnant women. Conclusion Serum TF and TFPI have a close relationship with the formation of placenta,and can be used as a sensitive indicator for the assessment of placenta function in PE,and has a significant value in early diagnosis and prevention of PE.