3种方法检测286例生殖道沙眼衣原体的分析

Analysis of three methods on detecting 286 cases of Chlamydia trachomatis

目的分析比较酶联免疫吸附试验(ELISA)、核酸扩增聚合酶链反应(PCR)荧光法和免疫层析法在检测泌尿生殖系统沙眼衣原体(CT)感染中的应用效果。方法分别采用ELISA、核酸扩增PCR荧光法和免疫层析法检测泌尿生殖系统的CT抗原,同时用细胞培养法作为"金标准"。结果共检286例标本,ELISA、核酸扩增PCR荧光法和免疫层析法的特异性和灵敏度分别为99.1%和95.5%;98.2%和97.0%;99.5%和73.6%。结论 ELISA法与核酸扩增PCR荧光法具有较一致灵敏度和特异性,均可为临床治疗和监测性病提供可靠的依据;免疫层析法虽然操作简便,但灵敏度较差。

Objective To analyze and compare the application performance of enzyme-linked immunosorbent adsorption test （ELISA）, nucleic acid amplification PRC fluorescence method and immune chromatography method on detecting Chlamydia trachomatis （CT） in the urogenital systems. Methods We detected CT in the urogenital sys tems by using ELISA,PCR fluorescence method and immune chromatography method respectively,and used cell culture as the ＂ gold standard＂ simutaneously. Results 286 cases of specimens were detected. The specificities of ELISA,PCR fluorescence method and immune chromatography method were 99.1%,95.5% and 98.2% respectively,and the sensitivities were 97. 0%,99. 5% and 73. 6% respectively. Conclusion ELISA and PCR fluorescence method are more consistent in sensitivity and specificity, both providing reliable basis for clinical treatment and sexually transmitted diseases. Immune chromatography method is less sensitive in spite of its simple operation.