7种检测系统测定新鲜血浆葡萄糖的比对分析

Comparative analysis on seven detection systems for testing of fresh plasma glucose

目的通过不同检测系统测定血糖(Glu)的方法比对,探讨不同检测系统间血糖的偏倚是否符合临床质量要求。方法以可溯源的OLYMPUS AU2700检测系统1为目标检测系统,用电化学法、干式化学法、透射比色法,应用本院共7种不同检测系统对新鲜血浆标本50例的血糖进行比对检测。结果各检测系统测定Glu的变异系数均小于5.0%;各系统间的相关系数均大于0.975;7个系统间总体差异无统计学意义(P>0.05);以可溯源的检测系统1为目标检测系统进行比对,在3个医学决定水平上,检测系统3、检测系统4和检测系统7超过T±5%范围,检测系统2、检测系统5和检测系统6均未超过T±5%范围。结论部分检测系统测定Glu的结果存在偏倚,对其实施整改措施后,结果具有可比性。

Objective To evaluate comparability of different detection systems for determination of blood glucose through bias estimation and method comparison,and ensure the clinical quality requirements.Methods Compared with OLYMPUS AU2700 dection system,50 cases of fresh blood plasma samples were detected glucose with different detcetion systems,including electrochemical method,dry chemical method and spectrophotometric method.Results The variation coefficient of different detection systems was less than 5.0%;The correlation coefficient between the various systems was more than 0.975;The overall difference between systems was not statistically significant（P0.01）;Compared with a traceable target detection system 1,Detection system 3,detection system 4,and detection system 7 exceeded the range of T ± 5%,Detection system 2,detection system 5,and detection system 6 were in the range of T ± 5% in the three medical decision levels.Conclusion There are bias in part of the different detection systems for glucose detection results,but the results are comparable through corrective measures.