鼠疫菌感染兔血清中的10种重要抗体的检测分析

Detection and analysis of ten important antibodies in sera of rabbits with Yersinia pestis infection

目的对鼠疫感染兔血清中的10种重要抗体(Ab)进行检测,寻找除F1外的其他可能用于鼠疫菌感染诊断的新靶标。方法利用一种新的基于上转发光法的十通道免疫层析检测技术(TC-UPT-LF)和另一种经典的间接酶联免疫吸附试验(ELISA),同时检测了54份抗F1-Ag抗体阳性的鼠疫菌感染兔血清样品,对样品中10种靶标抗体的阳性率进行了综合分析。结果在54份鼠疫菌感染兔血清样品中抗体检出阳性率较高的有3个,依次为F1-Ab(100%)、YPO1089-Ab(75%)、YopD-Ab(72%),其余7个靶抗体阳性率都低于50%。结论在鼠疫感染的诊断中,除现有的F1外,YopD极有可能作为新的辅助诊断靶标。

Objective To detect ten important antibodies in sera of rabbits with Yersinia pestis（Y, pestis） infection and to find new potential diagnostic markers besides F1 for the diagnosis of Y. pestis infection. Methods Ten target antibodies in 54 cases of serum specimens from rabbits with plague were detected by new lateral flow assay of ten-channel up-converting phosphor technolo gy-based lateral flow assay（TC UPT-LF） and classical indirect ELISA simultaneously and positive ratios of the ten target antibodies were aggregate analyzed. Results There were three antibodies with high positive ratio, including F1 （100 %）, YPO1089 （75 %） and YopD（72%）, while positive ratios of the other seven antibodies were less than 50%. Conclusion YopD could be a new potential diagnostic marker for Y. pestis infection besides F1.