同型半胱氨酸对脐静脉内皮细胞凋亡及活性氧生成的影响

Effect of homocysteine on the apoptosis and reactive oxygen species production of human umbilical vein endothelial cells

目的探讨同型半胱氨酸(Hcy)对人脐静脉内皮细胞(HUVECs)的损伤作用及机制。方法培养HUVECs,Hcy以不同浓度(0、0.01、0.1、1.0、3.0、5.0 mmol/L)和不同作用时间(3、6、12、24 h)进行干预。采用流式细胞仪检测细胞凋亡率及活性氧。结果随着Hcy浓度增高或作用时间延长,HUVECs凋亡率、活性氧生成均明显升高,差异均有统计学意义(P<0.01)。结论 Hcy能诱导内皮细胞凋亡,且其作用呈浓度和时间的依赖性。

Objective To investigate the effect of reactive homoeysteine（Hey）on the apoptosis and reactive oxygen species （ROS）production of human umbilical vein endothelial cells（HUVECs）. Methods HUVECs were cultured and treated with Hey in different concentration（0,0.0 1, 0.1,1.0,3.0,5.0 mmol/L） within different duration（3,6,12,24 h）. The presence of apoptosis and ROS were detected by flow cytometry. Results The apoptotic rate and ROS production increased signifcantly（P〈0.01） with the increase of the Hey concentration and treated duration. Conclusion Hcy could induce the the apoptosis of HUVECs, with the dependence of Hcy concentration and treated duration.