解毒化瘀颗粒对急性肝衰竭小鼠肝脏TNFRⅠ与TNFRⅡ表达的影响

Effects of Jiedu Huayu Particles on Expression of TNFR Ⅰ and TNFR Ⅱ in Acute Liver Failure Mice

目的:观察解毒化瘀颗粒对急性肝衰竭小鼠肝脏TNFRⅠ与TNFRⅡ表达的影响,探讨其拮抗肝衰竭的作用机制。方法:采用D-氨基半乳糖联合内毒素腹腔注射法构建小鼠急性肝衰竭动物模型,并分别予解毒化瘀颗粒、乳果糖、安宫牛黄丸干预治疗,HE染色观察肝脏病理改变,TUNEL法检查肝细胞凋亡率,免疫组化检测小鼠肝脏TNFRⅠ与TNFRⅡ的表达。结果:解毒化瘀颗粒干预组肝组织凋亡、坏死程度轻于模型对照组,免疫组化检测的肝脏TNFRⅠ表达量少于模型对照组(P<0.05),TNFRⅡ表达多于模型对照组(P<0.05)。结论:解毒化瘀颗粒可通过下调肝组织TNFRⅠ的表达、上调TNFRⅡ的表达途径抑制肝细胞凋亡,延缓肝脏坏死的进展。

Objective ： To observe the particle Jiedu Huayu liver of mice with acute liver failure and TNFR Ⅰ and TNFR Ⅱ expression, and to explore the role of the antagonistic mechanisms of liver failure. Methods ： D-galaetosamine intraperitoneal injection of the joint construction of endotoxin in acute liver failure animal model in mice, and were given Jiedu Huayu particles, laetulose, Angong Niuhuang intervention, HE staining of liver pathological changes, TUNEL method Check the rates of hepatocyte apoptosis, liver immunohistochemistry TNFR I and TNFR II expression. Results ： The intervention group Jiedu Huayu particles liver apoptosis, necrosis in model control group, the liver detected by immunohistochemistry the expression of TNFR I less than model group （P〈0.05）, TNFR Ⅱ expression than the model control group （P〈0.05） .Conclusion ： The particles can be reduced Jiedu Huayu TNFR I expression in liver tissue ,increased expression of TNFR Ⅱ inhibition of liver cell apoptosis pathway, delaying the progress of liver necrosis.