雷公藤甲素诱导大鼠肾上腺皮质细胞凋亡研究

A Study on Apoptosis in Adrenocortical Cells Induced by Triptolide

目的:了解雷公藤甲素诱导大鼠肾上腺皮质细胞凋亡的发生。方法:体外分离培养大鼠肾上腺皮质细胞,以蛋白因子添加素V和碘化丙啶(PI)联合标记,流式细胞仪检测,观察雷公藤甲素诱导细胞凋亡的特征。结果:雷公藤甲素以时间和剂量依赖的方式诱导细胞凋亡,0、12.5、25、50、100和200μg/L雷公藤甲素处理细胞2h,凋亡率分别为1.67%、16.54%、32.53%、47.62%、57.12%及77.45%;100μg/L雷公藤甲素染毒0、0.5、1、2、3和4h,细胞凋亡率分别为1.12%、14.37%、35.26%、49.75%、62.65%及78.46%。结论:雷公藤甲素可诱导肾上腺皮质细胞凋亡。

Objective ： To learn apoptosis induced by Triptolide in adrenocortical cells of rat. Methods ： Adrenocortieal cells of rat were dispersed and primarily in vitro, features of apoptotie ceils were observed using combined labeling with annexin-V and propidium iodide （ PI ） and flow cytometry. Results ： Apoptosis rate of adrenocortieal cells increased with dose （ 0-200 μg/L for 2h, from 1.67% to 77.45% ） or duration （ 100μg/ L for 0-4h, from 1.12% to 78.46% ） of Triptolide. Conclusions ： Apoptosis can be induced by Triptolide in adrenocortical cells.