1个水稻产量QTL区段及品系特异性重复序列的比较分析

Comparative analyses of a rice yield QTL region and variety-specific repetitive sequences

为克隆1个水稻产量QTL,对遗传作图亲本珍汕97和明恢63物理图谱上与该QTL相对应的区段进行了比较分析,关闭了2个物理图谱在该区段的物理缺口,对该区段珍汕97和明恢63 BAC末端序列分别与日本晴参考序列之间的预测单核苷酸多态性SNP位点进行了检验,发现大部分预测SNP序列正确,而有一部分预测SNP为BAC末端测序错误。验证了的SNP将是基因和QTL克隆的重要分子标记。同时对代表性的推测水稻中花11基因组特有重复序列进行了验证。

To clone a rice yield QTL,a comparative analysis on the region of the physical maps derived from rice varieties Zhenshan 97 and Minghui 63, the two parents for mapping the QTL, was performed. Physical gaps in this region of the two physical maps were closed. Putative SNPs of this region between Zhenshan 97 BAC end sequences and Nipponbare reference sequence, and that between Minghui 63 BAC end sequences and Nipponbare reference sequence were validated. Results showed that most putative SNPs are correct but some are from errors of BAC end sequence. The confirmed SNPs will be used as important markers in the positional cloning of gene or QTL. Furthermore, representative putative Zhonghua 11 specific repetitive sequences found in our laboratory through Zhonghua 11 BES analysis were comparatively analyzed via Southern hybridization. The results will be helpful in cloning of the rice yield QTL and realizing the evolution of rice renetitive sequences among different rice varieties.