摘要
采用PCR-SSCP技术对大通牦牛、甘南牦牛和天祝白牦牛(共277头)肌肉抑制素基因(MSTN)内含子2的部分序列进行了多态性研究,分析该基因与牦牛生长性状的相关性。结果表明,牦牛MSTN基因内含子2存在2个等位基因和3种基因型。在该基因座上,甘南牦牛、天祝白牦牛呈Hardy-Weinberg平衡状态,大通牦牛呈不平衡状态。3种基因型与牦牛部分生长性状的最小二乘法分析表明,MSTN基因内含子2对成年牦牛胸围、体质量、胸围指数、体长指数和肉用指数均显著相关(P<0.05),而不同基因型的牦牛体高、管围、体长和管围指数差异不显著。初步推断牦牛MSTN基因内含子2可作为牦牛标记辅助选择的遗传标记之一。
The PCR-SSCP polymorphism of myostation (MSTN) gene sequence of intron 2 and its correlation with growth traits were investigated in 277 individuals from one cultivated yak breeds (Datong yak) and two native breeds(Gannan and Tianzhu White yak). The results showed that there were two alleles and three genotypes in the locus of MSTN gene intron 2. They were at Hardy-Weinberg equilibrium state in this locus while Datong yak obviously was unequilibrium. The least square analysis showed that the different genotype of MSTN intron 2 had a significant effect on partial traits of growth including heart girth, body weight, index of chest circumference, index of body slanting length, beef performance index(P〈0.05). However the genotype did not affect body height, cannon circumference, body slanting length,index of cannon circumference significantly(P〉0. 05). In conclusion, the mutation of MSTN intron 2 could be used as the molecular genetic marker for yak breeding.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2011年第3期285-289,共5页
Journal of Huazhong Agricultural University
基金
国家"863"项目(2008AA10Z137)
中央级公益性科研院所基本科研业务费专项(BRF100101)
现代农业(肉牛)产业技术体系专项(MATS-BeefCattleSystem)
甘肃省重大科技专项(0801NKDA036)