摘要
目的 探讨 αｓｙｎｕｃｌｅｉｎ 基因已知突变与散发性帕金森病人的关系。方法 分离 Ｐ Ｄ组和对照组的外围血基因组 Ｄ Ｎ Ａ，采用 αｓｙｎｕｃｌｅｉｎ 基因特异引物对其第四外显子进行 Ｐ Ｃ Ｒ 扩增，扩增产物用 Ｔｓｐ４５Ⅰ酶切鉴定。结果 两组 Ｐ Ｃ Ｒ 均可扩增出 ２１６ ｂｐ 的片段， Ｐ Ｃ Ｒ 产物不能被 Ｔｓｐ４５Ⅰ酶切。结论 αｓｙｎｕｃｌｅｉｎ 基因已知点突变与散发性帕金森病可能无明确关系。
Objective To study the relationship betw een one m issense m utation of alpha synuclein gene andsporadic Parkinson's disease( P D). M ethods The genom ic D N A w as isolated from peripheralleukocytes of P D patients,or the norm al controls,and the positive control respectively. P C R experim ents w ere carriedout. Then P C R products w ere digested w ith Tsp45 I. Results The fragm ent of 216 bp w as am plified in tw ogroups,but their products could not be digested w ith Tsp45 I. Conclusion These results indicated that them utation identified in alpha synuclein gene for fam ilial P D w as unlikely to account for sporadic P D.
