酶法提取植物挥发油的研究

APPLICATION OF CELLULASE TO TREAT PLANT FOR INCREASING YIELD OF THE VOLATILE

植物挥发油的提取率与提取方法、操作步骤、预处理方法等有很大差异，为了提高天然植物挥发油提取率，本实验利用了在反应过程中分批添加底物的酶解方法，破坏植物细胞壁；并对分批添料的作用机理做了进一步的讨论．研究表明：分批添料的方法使菊花挥发油的提取率提高到０．４５％，为常规法的２．８倍，用ＧＣ／ＭＳ法从菊花挥发油中分离并确认出４８种化学成分。

In this paper the study on the methods of exacting the volatile from dendranthema.The results show that:the cellulase has ablities to improve the yield of and chang the relative contents of contituents in the volatile oils.Dendranthema was treated with cellulase,sample at 50 ℃ pH4.5 cultured for 24 h,theyield of the volatile was inereased then the control.The enzymatic hydrolysis in the style of different feeding methods in order that the yield of volafile oils.