葎草不同部位中木犀草素-7-O-β-D-葡萄糖苷和大波斯菊苷的含量测定

Determination of Luteolin-7-O-β-D-glucoside and Cosmosiin of Different Parts in Humulus Scandens

目的测定葎草的根、茎、叶、叶柄不同部位所含的木犀草素-7-O-β-D-葡萄糖苷和大波斯菊苷的含量。方法采用HPLC法测定葎草中木犀草素-7-O-β-D-葡萄糖苷和大波斯菊苷的含量,色谱柱为DikmaDiamonsil C18柱,以甲醇-0.2%磷酸溶液（45∶55）为流动相,流速为1.0mL.min-1,柱温为35℃,检测波长为350nm。结果木犀草素-7-O-β-D-葡萄糖苷在0.05~0.40μg的范围内与峰面积积分值成良好的线性关系（r=0.9999）;大波斯菊苷在0.072~0.36μg的范围内与峰面积积分值成良好的线性关系（r=0.9997）。根与叶中主要含有木犀草素-7-O-β-D-葡萄糖苷和大波斯菊苷,且叶中的含量最高。茎与叶柄中含有较多的其他成分。结论该方法可用于葎草中木犀草素-7-O-β-D-葡萄糖苷和大波斯菊苷的含量测定。葎草的叶与其他部位应分别药用。

Objective To determine the content of Luteolin-7-O-β-D-glucoside and Cosmosiin of roots,stems,leaves and petioles in Humulus Scandens.Methods The Dikma Diamonsil C18 column was used.The mobile phase consisted of methanol-0.2%phosphoric acid（45∶55）.The flow rate was 1.0mL·min-1.The column temperature was 35℃,and the UV detection wavelength was 350nm.Results Luteolin-7-O-β-D-glucoside and Cosmosiin showed a good linear range within 0.072~0.36μg（r=0.9999） and 0.05~0.40μg（r=0.9997） respectively.Roots and leaves mainly contained Luteolin-7-O-β-D-glucoside and Cosmosiin.The content was the highest in leaves.The stems and petioles contained more other components.Conclusion The method can be used to determine the content of Luteolin-7-O-β-D-glucoside and Cosmosiin by HPLC.The leaves and stems should be separately medicined.