HPLC法测定多维生素糖浆中D-泛醇含量

Determination of D-panthenol in Alvityl Syrup by HPLC

目的建立HPLC-UV法测定多维生素糖浆中D-泛醇的含量。方法采用Agela AQ-C18柱;流动相A为0.05mol.L-1磷酸二氢钾-乙腈（99∶1）;流动相B为0.05mol.L-1磷酸二氢钾-乙腈（80∶20）;梯度洗脱;流速为1.0mL.min-1;检测波长为214nm。结果 D-泛醇的浓度在0.02-0.4mg.mL-1范围内线性关系良好（r=1.0000,n=5）;平均回收率为99.8%（RSD=0.4%,n=9）。结论本分析方法快速、灵敏,分离度良好,结果准确可靠,可作为多维生素糖浆的质量控制项目之一。

Objective To develop a HPLC-UV method for determination of D-panthenol in Alvityl Syrup.Methods After being diluted with pure water,samples were separated by HPLC on Agela AQ-C18 column with mobile phases of 0.05mol·L-1 potassium dihydrogen phophate water solution-acetonitrile（99∶1） and 0.05mol·L-1 potassium dihydrogen phophate water solution-acetonitrile（80∶20）.The mobile phases were gradient eluted.The flow rate was 1.0mL·min-1.The detection wavelength was at 214nm.Results Calibration curve for D-panthenol was linear over the range of 0.02-0.4mg·mL-1（r=1.0000,n=5）.And the average recovery was 99.8%（RSD=0.4%,n=9）.Conclusion The assay is proved to be sensitive,accurate and convenient with good resolution.It can be applied to control the quality of Alvityl Syrup.