大豆种皮过氧化物酶在木素降解与苯酚聚合中的应用

Application of Soybean Hull Peroxidase in Lignin Degradation and Phenol Polymerization

以大豆种皮过氧化物酶（Soybesnhullperoxidase，SHP，E．C．1.111．7）替代水煮过氧化物酶（LigninperoxidaseLiP）和锰过氧化物酶（Manganese－dependentperoxidase，MnP），以工业木素（木素磺酸盐和黑液木素）及天然木素（杉木木素）为材料，研究了SHP在正常催化条件下对木素的氧化作用。结果表明，SHP在水相系统中对工业木素和天然木素虽无降解作用，但在微水有机相中SHP对天然木素有部分解聚作用。利用SHP在水相体系中催化的酚聚合反应对含酚废水进行处理，则发现水相中SHP稳定性好，lmol／L的SHP和lmnlol／L的H202在15mitt内对终浓度为lmmol／L宋酚溶液的酚去除率达98％以上，提示SHP作为一种较理想的氧化还原酶用于含酚废水处理值得深入研究。

The oxidation of lignins including sodium lignosuifonate, black liquid lignin and sprucelignin by soybean hull peroxidase (E. C. l. 11 l. 7 ) instead of lignin peroxidase or manganese -dependent peroxidase in normal catalytic cycle was studied. The results showed that none of thelignins mentioned above was degraded by soybean hull peroxidase in water - phase system.However,spruce lignin was partially degraded by soybean hull peroxidase in micro - waer -solvent phase system. In addition, SHP was stable in water - phase system. l pmol/L SHP plus lnunol/L H2o2 catalyzed polymerization 0f phen01 in an l rnrnol/L phenol solution by more than98%. This indicats that SHP could be an ideal peroxidase to be used in the treatment ofwastewater containing phenolic compounds.