高效液相色谱-二极管阵列检测方法结合交替三线性分解算法快速定量测定中药黄芩中的黄芩素和汉黄芩素

Simultaneous Determination of Baicalein and Wogonin in Chinese Medicinal Plant Scutellaria Baicalensis Using HPLC-DAD Coupled with Alternating Trilinear Decomposition

利用高效液相色谱-二极管阵列检测(HPLC-DAD)方法结合化学计量学基于交替三线性分解算法的二阶校正方法,在未知基体干扰共存下,同时测定了中药黄芩中的黄芩素和汉黄芩素的含量。当体系组分数选取为3时,测得黄芩素和汉黄芩素的平均回收率分别为104.6±4.3%和100.7±7.4%。该方法不仅能同时给出2种物质的相对色谱图和相对光谱图,还能同时测定未知干扰存在下的2种物质的浓度。

This paper proposed a novel method for simultaneous determination of baicalein and wogonin in Chinese medicinal plant Scutellaria baicalensis by using high performance liquid chromatography with photodiode-array detection（HPLC-DAD） coupled with second-order calibration method based on the alternating trilinear decomposition algorithm,especially in the presence of matrix interferences.When the core consistency diagnostic suggested N was set to 3,the average recoveries of baicalein and wogonin were 104.6±4.3% and 100.7±7.4%,respectively.