摘要
为了制备抗莱克多巴胺多克隆抗体,试验通过连接剂butane-1,4-diol diglyciydyl ether把莱克多巴胺和载体蛋白BSA和OVA偶联成免疫抗原和包被抗原,免疫新西兰大白兔,饱和硫铵沉淀方法纯化抗体,间接ELISA法检测抗血清效价。结果通过免疫兔获得了抗莱克多巴胺的多克隆抗体。经ELISA测定,其效价为1∶12800。
The RCT（Ractopamine） was coupled with BSA and OVA to produce immunogen and envelope antigen by using coupling agent butane-1,4-diol diglyciydyl ether,respectively.The polyclonal antibodies was obtained by immuning New Zealand rabbits with BSA-RCT and purified by saturated ammonium sulfate precipitation.The indirect ELISA method was used to detect its antiserum titer.The results showed that the polyclonal antibodies against RCT had been obtained from immunized rabbits and the titer of the polyclonal antiserum was 1∶12 800.
出处
《湖北农业科学》
北大核心
2011年第9期1836-1837,1841,共3页
Hubei Agricultural Sciences
基金
江苏省农业三项工程项目[sx(2009)177]
