摘要
目的探讨有氧运动联合膳食干预对2型糖尿病大鼠骨骼肌AMPK活性和蛋白含量的影响。方法 6周龄雄性SD大鼠62只,随机抽取8只大鼠作为正常对照组(C组,n=8),喂以标准普通饲料。其余54只在喂饲高糖高脂膳食的基础上,腹腔注射小剂量的链脲佐菌素(STZ),建立2型糖尿病动物模型。然后将2型糖尿病大鼠随机分成4组:DM对照组(DM,n=9)、DM+运动锻炼组(DME,n=10)、DM+膳食控制组(DMD,n=10)、DM+运动锻炼+膳食控制组(DMED,n=10)。DM组大鼠继续喂饲高脂高糖饲料,不进行运动锻炼;运动锻炼采用每天进行60 min的无负重游泳运动,每周6次;膳食控制采用与DM组等量的标准普通饲料。12周后,检测各组大鼠FPG、FINS、骨骼肌中AMPK含量和活性。结果 (1)与C组相比,DM组大鼠FPG显著升高(P<0.01),FINS显著降低(P<0.01)。双因素方差分析显示,与DM组相比,DME组FPG显著性降(P<0.05),FINS虽有上升,但没有显著性差异(P>0.05);DMD组FINS显著升高(P<0.05),但FPG没有显著性变化(P>0.05);运动联合膳食控制对糖尿病大鼠FPG、FINS均无显著性交互作用。(2)与C组相比,DM组大鼠骨骼肌中AMPK含量和活性均显著性下降(P<0.01,P<0.05);双因素方差分析显示,与DM组相比,DME组骨骼肌中AMPK含量和活性均有显著升高(P<0.05、P<0.01)。DMD组骨骼肌中AMPK含量和活性均无显著性变化。运动联合膳食控制对糖尿病大鼠骨骼肌中AMPK含量和活性均无显著性交互作用。结论 (1)研究成功复制了2型糖尿病大鼠模型,而有氧运动锻炼对2型糖尿病大鼠具有显著的干预作用。(2)2型糖尿病大鼠骨骼肌中AMPK含量和活性显著降低,有氧运动锻炼不仅有效地提高2型糖尿病大鼠骨骼肌AMPK含量,而且可显著增加AMPK活性,对改善2型糖尿病机体的糖脂代谢具有非常重要的作用,但膳食控制对其作用不明显。
Objective To research the effects of aerobic exercise and dietary control on AMPK of skeletal Muscles in mice with type 2 diabetes. Method 62 male SD mice were randomly divided into control group and DM model group,they are fed by normal chow and high glucose and fat diet respectively. After the DM model was established, the DM model mice were randomly divided into four groups as diabetes control group( DM), diabetes and exercise group( DME), diabetes and dietary control group(DMD) ,diabetes and exercise and dietary control group(DMED). Exercise training used is 60 minutes' unload swimming,6 times per week,for 12 weeks. The serum glucose concentration was measured by method of glucose oxidase, the serum insulin concentration was measured with RIA method. The protein content of AMPK in skeletal muscles was measured by ELISA, the activity of AMPK was measured by radioisotope method. Results ( 1 ) Compared with control group,the FPG was significantly increased(P 〈0.01 ) ,and the FINS in DM group was significantly decreased(P 〈0.01 ). Through the two - factor analysis of variance, compared with DM group, the FPG in DME group was significantly reduced( P 〈0. 01 ) ,the FINS in DME group was increased, but with no significance, the FINS in DMD group significantly increased but the FPG was of no significance( P 〉 0. 05 ), exercise and dietary control were not interactive on the FPG and FINS. (2)Compared with control group,the AMPK content and activity in DM group significanlly decreased( P 〈 0. 01, P 〈 0.05). Through the two -factor analysis of variance, the AMPK content and activity in DME group significantly increased (P 〈 0. 05, P 〈 0. 01 ). the AMPK content and activity in DMD group were no difference( P 〉 0. 05 ), exercise and dietary control were also no interaction on them. Conclusion ( 1 )The model of type 2 diabetic rats were successfully replicated in This study,and aerobic exercise had a significant intervention effect on type 2 diabetic rats. (2) AMPK content and activity in skeletal muscle of type 2 diabetes were significantly decreased, aerobic exercise would not only effectively increase AMPK content, but also significantly increase AMPK activity in the skeletal muscle of type 2 diabetes,which had a very. important role on the Glucose and lipid metabolism in type 2 diabetes, but the dietary control had not obvious effect on it.
出处
《西安体育学院学报》
CSSCI
北大核心
2011年第3期344-348,共5页
Journal of Xi'an Physical Education University
基金
扬州大学科技创新培育基金项目(2009CXJ009
2010CXJ018)