L-精氨酸对大鼠肾缺血再灌注损伤HSP70表达的影响

Effect of L-arginine on the Heat Shock Protein 70 of Renal Ischemia-reperfusion Injury in Rats

目的:探讨左旋精氨酸(L-arg)对大鼠肾缺血再灌注损伤(RIRI)HSP70表达的影响。方法:健康雄性SD大鼠48只,随机分为6组:假手术(Sham)6 h、24 h组;缺血再灌模型(IR)6 h2、4 h组和L-精氨酸(L-arg)6 h2、4 h组。IR组和L-arg组采用夹闭双侧肾动、静脉45 min后松夹再灌的方法制备RIRI模型,L-arg组于夹闭前和再灌前15 min分别尾静脉注射L-arg 0.2 mL(100 mg/kg),于再灌后6 h和24 h测定血清肌酐(Scr)、一氧化氮(NO)及尿NAG酶(UNAG)含量,观察肾组织形态学改变和肾皮质HSP70表达。结果:缺血再灌注后6 h和24 h,大鼠肾功能和肾小管上皮细胞明显受损,Scr和UNAG含量均明显高于假手术组(P<0.01),血清NO及肾皮质细胞HSP70表达明显增加。尾静脉注射L-arg可明显改善RIRI大鼠肾功能,减轻肾损伤,并明显增加血清NO及肾皮质细胞HSP70表达(P<0.05)。结论:L-arg可通过增加体内NO水平,提高肾小管上皮细胞HSP70表达,对大鼠RIRI起到一定的保护作用。

Objective：To investigate the possible role of L-arginine in the pathogenesis of the heat shock protein 70 in renal cortex cells of renal ischemia-reperfusion injury（RIRI） in rats.Methods：48 SD rats were divided into 6 groups randomly：Sham 6 h,24 h groups,IR 6 h,24 h groups and L-arg 6 h,24 h groups.The model of RIRI was induced by bilateral clamping the renal artery and vein for 45 minutes followed by reperfusion.L-arg 100 mg/kg was injected via the tail vein before ischemia and 15 min before reperfusion.Serum creatinine（Scr） and urea N-Acety-P-beta-Glucosaminidase（UNAG） were measured for assessment of renal function.Nitric oxide（NO2-/NO3-） and renal histological changes were tested for determination the degree of renal injury.Flow cytometer was made for estimate the contents of HSP70 in renal cortex cells.Results： Contents of Scr and UNAG were significantly increased in RIRI rats（P0.01）,serum NO levels and the expression of HSP70 were both markedly increased in RIRI rats.L-arg might significantly decrease the contents of Scr and UNAG.L-arg also could significantly enhance the expression of serum NO and the HSP70 levels of renal cortex cells consequently and attenuated the renal histological injury in RIRI rats.Conclusion：L-arg has beneficial effects on renal dysfunction in RIRI rats through production NO and enhances the HSP70 expression of the renal cortex cells.