摘要
以樱桃番茄叶片为外植体在附加6-BA 2.0mg.L+IAA 0.1mg.L的MS培养基上诱导体细胞胚发生,研究了樱桃番茄体细胞胚胎发生过程中抗氧化酶活性和某些生理参数的变化。结果表明,在樱桃番茄体细胞胚发生过程中,抗氧化酶活性变化表现为SOD活性在培养第7天和第21天出现2次高峰,分别为73.69U.g-1FW和77.76U.g-1FW;POD活性表现为先降后升的变化趋势,在培养21d时达到最低值,为0.6317U.g-1min-1FW;CAT活性变化幅度不大,在培养28d时活性最大,为23.0798mg.g-1min-1;H2O2含量表现为先升后降,且在培养第14天出现高峰,为11.4522μmol.L-1 g-1 FW;丙二醛含量整体呈明显的下降趋势;IAA氧化酶活性变化趋势为升-降-升-降,在35d时IAA氧化酶活性最大,为737.0748μg(IAA)g-1FW h-1。生理参数的变化表现为生长量呈上升趋势;可溶性蛋白含量呈先降后升再降,且在第35天出现高峰,为0.7299mg.g-1FW;叶绿素含量整体呈现降-升-降-升的变化趋势,第28天出现高峰期,为1.5891mg.g-1FW;电导率的变化趋势为降-升-降-升,14d时达到最大值,为25.8%。据此认为,在樱桃番茄叶体细胞胚发生过程中,POD对体细胞胚的诱导起主导作用;SOD活性、H2O2含量与胚性细胞的发育密切相关;可溶性蛋白含量、细胞膜透性与体细胞胚发生密切相关;IAA氧化酶活性与愈伤组织形成和胚性细胞的分化密切相关。
The somatic embryogenesis was induced from the leaves of cherry tomato(Lycopersivon esculentum Mill.) on MS medium with 2.0mg·L-1 6-BA and 0.1mg · L-1 IAA,and the changes of antioxidant activities and some physiological parameters during somatic embryogenesis were analyzed.Results showed that the activities of antioxidant enzymes were as follows during somatic embryogenesis.Firstly,SOD activity of the callus presented two peaks of 73.69U· g-1 FW and 77.76U · g-1 FW on the 7th and the 21st cultured days,respectively.And POD activity decreased,then increased to its minimum(0.6317U·g-1 min-1 FW) on the 21st day.However,CAT activity had no significant change during the whole culture time and the highest value was 23.0798mg·g-1min-1 on the 21st day.At the same time,IAA oxidase activity had the highest value(737.0748μg(IAA) g-1 FWh-1) on the 35th day and the lowest value was 122.954 μg(IAA) g-1 FWh-1.Furthermore,MDA showed a significant downward trend overall,and H2O2 content increased till the 14th day(the highest value was 11.4522μmol·L-1g-1FW) and then decreased.Changes of the physiological parameters showed that the fresh weight of the callus gradually increased with the extension of culture time, and the changes of soluble protein content had a peak value 0.7299mg·g-1 FW on the 35th day.The highest value of chlorophyll content was 1.5891mg · g-1 FW on the 28th day.Besides,the change of electrical conductivity had a maximum(25.8%) on the 14th day.It indicated that POD played an important role in somatic embryogenesis of cherry tomato leaf explants.At the same time,soluble protein content and cell membrane permeability were correlated with process of somatic embryogenesis;the activities of SOD and IAA oxidase as well as H2O2 accumulation had regular changes,which closely related to the development of embryonic cells.
出处
《核农学报》
CAS
CSCD
北大核心
2011年第3期594-601,共8页
Journal of Nuclear Agricultural Sciences
基金
陕西省生物技术重点实验室项目(08JZ72)
陕西省教育厅专项科研资助项目(09JK746)
国家自然科学基金(31000144)
陕西省财政专项
西北大学自主创新类项目(10YZZ36)