杭州地区急性呼吸道感染患儿人博卡病毒的检测及分析

Detection and analysis of human bocavirus among children with acute respiratory tract infection in Hangzhou,Zhejiang

目的了解杭州地区人博卡病毒(HBoV)的流行情况和基因变异情况。方法对杭州地区160例儿童急性呼吸道感染患者咽拭子样本进行HBoV检测,并对阳性样本进行常见呼吸道病毒的检测,最终选取HBoV单一阳性的HZ1株和HZ2株进行VP1基因扩增,将获得的序列上传Genbank并与其他国家和地区的8株HBoV的VP1基因进行序列比对和分析。结果杭州地区HBoV的检出率为6.9%(11/160),与其他常见呼吸道病毒的合并感染率达到45.5%(5/11),HZ1株和HZ2株与其他国家和地区的8株HBoV的VP1基因相似性达到99%以上。结论研究证明杭州地区急性呼吸道感染患儿中存在HBoV的感染,与其他呼吸道病毒有较高的合并感染,从分离到的HZ1株和HZ2株来看,杭州地区流行的是ST2基因型,其外壳蛋白VP1基因变异很小。

Objective To study the prevalence and gene variation of human bocavirus（HBoV） among children with acute respiratory tract infection（ARTI）.Methods A method of polymerase chain reaction was applied to detect HBoV infection in swab specimens among children with ARTI.HBoV infected specimens were also tested for other respiratory viruses to evaluate the co-infection of different pathogens.The VP1 gene fragments were amplified and sequenced from the strains of HZ1 and HZ2,and were compared with the sequences of strains isolated from other 8 countries and areas.Results The positive rate of HBoV infection was 6.9%（11/160） for the clinical ARTI specimens.Five children had the co-infection of HBoV with other viruses.The homology of DNA sequences was very high（99%） among the strains of HZ1,HZ2 and strains isolated from other 8 countries and areas.Conclusion HBoV was an important pathogen singly or by co-infection with other respiratory viruses in the pediatric patients of ARTI in Hangzhou.