中央空调系统嗜肺军团菌传统细菌培养与荧光PCR结果比较

Comparison of culture and fluorescence PCR methods for Legionella pneumophila detection in central air conditioning systems

目的中央空调系统嗜肺军团菌传统细菌培养与TaqMan荧光定量PCR检测结果比较。方法对9家商场、宾馆、医院、办公楼公共场所中央空调系统的冷却塔水、生物膜、淤泥三环节样本进行军团菌检测,用传统细菌分离培养法分离鉴定,并用TaqMan荧光定量PCR法加以验证。结果本次对9家单位的水冷式中央空调系统三环节采样18份,其中传统细菌培养法检出4份军团菌,检出率为22.2%;冷却塔水、生物膜、淤泥检出率分别为22.2%、25.0%、20.0%。用荧光PCR法检出9份,检出率为50.0%;冷却塔水、生物膜、淤泥检出率分别为44.4%、50.0%、60.0%。结论金华市中央空调系统中存在嗜肺军团菌,荧光PCR检出率高于传统细菌培养法。

Objective To compare traditional culture and real-time TaqMan-based polymerase chain reaction（PCR） methods for the detection of Legionella pneumophila in central air conditioning system.Methods Nine stores,hotels,hospitals and offices were randomly selected for the study.Specimens were collected from the cooling water,the biofilm and the silt of central air conditioning systems.Legionella was tested by traditional culture,and was verified with real-time TaqMan-based PCR.Results Eighteen samples of water-cooled central air-conditioning systems were tested and four（22.2%） strains of Legionella pneumophila were isolated.The detection rates of Legionella pneumophila were 22.2%,25% and 20% respectively for the specimens collected from the cooling water,the biofilm and the silt.The method of real-time TaqMan-based PCR detected bacterial in 9（50%） specimens.The detection rates of Legionella pneumophila were 44.4%,50% and 60% respectively for the specimens collected from the cooling water,the biofilm and the silt.Conclusion Legionella pneumophila was existed in the central air conditioning systems in Jinhua city.The Legionella pneumophila detection rate was higher by real-time TaqMan-based PCR than by traditional culture.