中华苦荬菜总黄酮和总三萜提取工艺优化研究

Optimum Extracting Technology of Totals Flavonoids and Triterpenoids for Ixeris Chinensis(Thunb) Nakai by Orthogonal Text

以中华苦荬菜的总黄酮和总三萜为筛选指标,分别对索氏提取、超声波提取、微波提取设计正交试验,比较3种方法对有效成分的提取效率。每种方法得到优化整合方案,最后进行得率比较,优选最佳提取工艺。结果表明超声波提取法对总黄酮和总三萜提取得率最高,单次提取即可同时达到2种物质提取率的较好方法。其最佳条件为80%乙醇,在45℃条件下提取40 min。该法简便,提取率高,可用于快速提取中华苦荬菜中的有效成分。

Objective the optimal extraction technology of Ixeris chinensis（Thunb） Nakai studied using the content of totals flavonoids and triterpenoids as the indicators.Respective orthogonal text was carried on with microwave,ultrasonic wave extraction and soxhlet methods,and all the extraction effects were compared for the filtration of extracting technology.The result indicated that ultrasonic extraction had the advantage of higher efficiency.The optimal conditions were 80 % alcohol,the extractive time was 40 min,the extractive temperature was 45 ℃.The method was simple,efficient,and can be used to control the quality of Ixeris chinensis（Thunb） Nakai.