莽山烙铁头蛇粗毒的生物学活性

Study on the Biological Activity of Crude Venom From the Ermia Mangshanensis

分析了莽山烙铁头蛇粗毒对小鼠的半致死量,并采用膜片钳全细胞记录技术,研究了莽山烙铁头蛇粗毒对大鼠背根神经节神经元河豚毒素不敏感(TTX-R)钠通道、河豚毒素敏感(TTX-S)钠通道、低电压激活(LVA)电压依赖性钙离子通道和高电压激活(HVA)钙离子通道的作用。结果表明:粗毒对小鼠的半致死量为4.2 mg/kg,粗毒对钠通道和钙离子通道作用存在明显差异,10、100、1000 mg/L的莽山烙铁头蛇毒素对河豚毒素不敏感(TTX-R)钠电流有抑制作用,而100mg/L的莽山烙铁头蛇毒素对河豚毒敏感型(TTX-S)钠电流无明显影响;100、1000mg/L的莽山烙铁头蛇毒素对HVA型钙离子通道有抑制作用;1000mg/L的莽山烙铁头蛇毒素对LVA型钙离子通道电流峰值有抑制作用.

In this paper,the cell patch-clamp technique was used to study the crude venom on the effect of rat root ganglion neurons back TTX-R sodium channels,TTX-S-type sodium channels,Calciumion channels HVA type and Calciumion channels LVA type.The results showed that 10,100,1000 mg / L of venom significantly inhibited TTX-R sodium channel and the HVA-type calcium channel in a dose dependent manner;it also pointed out 100,1000mg / L of venom can inhibit the LVA type calcium channel current,while the TTX-S sodium channel type was not inhibited.