高效液相色谱法同时测定麻仁丸中大黄素、大黄酚、大黄酸、大黄素甲醚、芦荟大黄素的含量

Simultaneous determination of emodin,chrysophanol,rhein,physcion and aloe-emodin in Maren Wan by HPLC

目的建立麻仁丸中大黄素、大黄酚、大黄酸、大黄素甲醚、芦荟大黄素的含量测定方法。方法采用奥泰ALLTIMA-C18色谱柱（4.6 mm×250 mm,5μm）,以甲醇（A）-0.1%磷酸溶液（B）为流动相,梯度洗脱[09min,60%A;920 min,60%→80%A;2045 min,80%A];流速：1.0 mL.min-1;柱温：30℃;检测波长254 nm。结果大黄素、大黄酸、大黄酚、大黄素甲醚、芦荟大黄素线性范围分别为在9.0881.72、8.475.6、13.42120.7、7.5668.04、8.273.8μg.mL-1,与峰面积线性关系良好,平均回收率分别为99.1%、99.6%、99.4%、99.8%、99.2%,RSD分别为2.0%、1.8、3.2%、1.1%、1.5%。结论本方法可作为麻仁丸中大黄素、大黄酚、大黄酸、大黄素甲醚、芦荟大黄素含量测定的一种准确、灵敏、可行的方法。

Objective To establish an HPLC method for the simultaneous determination of emodin,chrysophanol,rhein,physcion and aloe-emodin in Maren Wan.Methods ALLTIMA-C18（4.6 mm×150 mm,5 μm） was used with methanol（A）-0.1% phosphoric acid solution（B） as the mobile phase in gradient elution[0-9 min,60%A;9-20 min,60%-80%A;20-45 min,80%A].The flow rate was 1.0 mL·min-1,and the column temperature was 30℃.The detection wavelength was 254 nm.Results The linear range of emodin,rhein,chrysophanol,phy scion,and aloe-emodin was 9.08-81.72,8.4-75.6,13.42-120.7,7.56-68.04,and 8.2-73.8 μg.mL-1,the average recovery was 99.1 %,99.6 %,99.4 %,99.8 %,and 99.2 %,with RSD 2.00 %,1.75 %,3.20 %,1.03 %,and 1.45 %.Conclusion The method is accurate,sensitive and feasible with good reproductivity,which can be used for the quality control of emodin,chrysophanol,rhein,physcion and aloe-emodinin Maren Wan.