深海微生物絮凝剂HBF-3的剂型研究

Study on Formulation of the Deep-Sea Microbial Flocculant HBF-3

对深海微生物絮凝剂HBF-3的剂型进行了研究。结果表明,以5%蔗糖为保护剂,采用冷冻干燥技术将发酵液制成固体剂型,冻干的HBF-3能够迅速复溶于水,絮凝活性为82.4%,维持了较高的絮凝活性;采用旋转蒸发浓缩将发酵液制成液体剂型,HBF-3浓缩液按浓缩倍数稀释后的絮凝活性与初始发酵液相比,变化不大;在液体剂型HBF-3中添加1.0 g.L-1山梨酸钾,于4℃、28℃、37℃、50℃储存一个月后能够保持90%以上的絮凝活性。

The formulation of the novel microbial flocculant HBF-3 was studied in this paper.The supernatant of the fermentative culture was made into solid formulation by freeze-drying with 5% sucrose as a cryoprotectant.The lyophilized power of HBF-3 could be easily rehydrated and therefore kept a flocculating activity as high as 82.4%.The liquid formulation was obtained through straightforward rotary evaporation process,which could mainly maintain the flocculating activity unchanged when diluting the concentrate to original concentration.The results also demonstrated that HBF-3 was thermostable.More importantly,the liquid formulation HBF-3 added 1.0 g·L-1 sorbic acid potassium maintained above 90% flocculating activity even one month later when stored at 4 ℃,28 ℃,37 ℃,and 50 ℃,respectively.