黄芪对糖尿病肾病大鼠尾加压素Ⅱ表达的影响

Effect of Astragalus on Expression of Urotensin Ⅱ in Renal Tissues of Rats with Diabetic Nephropathy

目的探讨黄芪对糖尿病肾病大鼠表达的影响。方法采用高糖高脂饮食和链脲佐菌素(STZ)腹腔注射法建立大鼠糖尿病肾病模型。将大鼠分为正常对照组(NC,n=10)、糖尿病肾病组(DN,n=10)、黄芪治疗组(HZ,n=10)(予黄芪注射液5 ml/kg灌胃)。于14周末处死动物,进行以下实验:测定血糖(BG)、胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)、血肌酐(Scr)、尿素氮(BUN)和尿蛋白定量(24 h UTP);免疫组织化学技术检测UⅡ蛋白表达,RT-PCR技术检测UⅡmRNA表达。结果与NC组相比,各模型组肾脏肥大指数(KI)、BG、TC、TG、Scr、BUN和24 h UTP明显升高,HDL明显降低,差异有统计学意义(P<0.01);HZ组的上述指标与DN组比较,则有明显降低,差异有统计学意义(P<0.05)。与NC组比较,DN组肾组织UⅡ表达增加,UⅡmRNA表达增加(P<0.01);与DN组比较,HZ组肾组织UⅡ表达明显降低,UⅡmRNA表达显著减少(P<0.05)。结论黄芪可治疗糖尿病肾病,其机制可能与降低肾组织UⅡ蛋白及其mRNA的过度表达有关。

Objective To investigate the effect of Astragalus on the expression of urotensin Ⅱ in renal tissues of rats with diabetic nephropathy.Methods Rat model with diabetic nephropathy was established by high-carbonhydrate and high fat diet and intraperitoneal injection of streptozotocin（STZ）.The rats were randomly divided into 3 groups that is normal control group（NC,n=10）,diabetic nephropathy group（DN,n=10）,Astragalus treated group（HZ,n=10）（fed to the Astragalus 5 ml/kg）.Animals were sacrificed at 14 weekends and under experiment： Determination of glucose,cholesterol,triglyceride,high-density lipoprotein cholesterol（HDL-C）,low density lipoprotein,serum creatinine,blood urea nitrogen and urinary albumin quantitative（24 h）.immunohistochemical techniques UⅡdetection protein expression,RT-PCR technology detection UⅡ mRNA expressions.Results Compared with NC group,each model group tentative KI,BG,TC,TG,Scr,BUN,24 h UTP increased significantly,HDL reduced significantly,and difference was statistically significant（P0.01）.The above indexes HZ group compared with the DN decreased obviously,there is a statistically significant difference（P0.05）.Compared with NC group the expression of UⅡand UⅡmRNA were increased significantly in renal tissues of DN group（P0.01）.However,the expression of UⅡand UⅡ mRNA in HZ group were significantly decreased in comparison with DN group（P0.05）.Conclusion Astragalus can be used to treat diabetic nephropathy and the mechanism may be related to down-regulating the expression of UⅡprotein and UⅡ mRNA.