摘要
目的研究枸杞多糖（LBP）对THP-1单核细胞源性巨噬细胞内内质网应激反应蛋白及氧化低密度脂蛋白（ox-LDL）含量变化的影响。方法培养THP-1单核细胞，加入佛波酯Qhorb0112-myristatc13-acetat，PMA）诱导分化成THP-1巨噬细胞，随后，加入不同浓度的LBP（207g／ml、507g／ml、1007g／ml、2007g／m1）孵育24h后收集细胞。用酶联免疫吸附法（ELIsA）检测细胞上清液中OX-LDL及内质网应激反应蛋白（Grp78，CHOP，Xbp-1）的含量变化。结果不同浓度的LBP孵育THP-1巨噬细胞，细胞中的OX-LDL与内质网应激反应蛋白的含量均降低。结论LBP可以降低OX-LDL及内质网应激反应蛋白的含量，对预防动脉粥样硬化的发生发展起到一定的作用。
Objective To study the effect of Lycium barbarum polysaccharides on expression of endoplasmic reticulum stress in macrophages derived from THP-1 monocytes.Methods THP-1 monocytes were induced into THP-1 macrophages by culturing with PMA.After exposure of the cultured THP-1 monocytes to LBP at increasing concentrations(0,20,50,100 and 200?g/ml) for 24 h.ELISA was used to determine the expression of Grp78,CHOP,Xbp-1 and ox-LDL in each group.Results THP-1 monocyte-derived macrophages were treated with different concentrations of LBP,we found that the expression of ox-LDL and Grp78,CHOP,Xbp-1 were decreased.Conclusion LBP may down-regulate the expression of Grp78,CHOP,Xbp-1 and ox-LDL.
出处
《医学信息》
2011年第11期3285-3287,共3页
Journal of Medical Information
基金
宁夏回族自治区区级大学生创新性实验获批项目（宁教函（20lo）381号）
