摘要
以第三代摩拉×本地奶水牛的新鲜乳为原料,对乳中过氧化物酶的最适温度、热稳定性、最适pH值、pH值稳定性以及光稳定性用ABTS进行测定。结果表明,乳中过氧化酶最适合温度在55℃附近,且在40℃以下稳定性较好;最适pH值在5.5附近,pH值为3.0～8.0都很稳定;日光灯照射条件下,酶的稳定性较差而避光条件下相对较好。这些研究将为南方以及广西地区水牛乳的保鲜提供了部分依据。
Fresh milk of Murrah×local third filial generation waterbuffalo was collected to analysis lactoperoxidase characteristics.The influences of temperature,pH and light on the lactoperoxidase activity were investigated with using 2,2＇-azino-bis（3-ethylbenzothiazoline-6-sulphonate） diammonium salt.The results showed that lactoperoxidase had a good stability below 40 ℃ and the most suitable temperature was 55 ℃ in the vicinity.The optimum pH for lactoperoxidase was around 5.5 and it had a high stability when pH is between 3.0 and 8.0.The enzyme had a poor stability under light condition,while a good stability when avoiding light.These researches will provid some basis for the freshing of waterbuffalo milk in South area of China as well as in Guangxi province.
出处
《中国乳品工业》
CAS
北大核心
2011年第6期4-7,共4页
China Dairy Industry
关键词
水牛乳
乳过氧化物酶
特性
waterbuffalo milk
lacperoxidase
lacperoxidase activity
