摘要
试验采用碳二亚胺(EDC)法将培氟沙星(PEFX)与载体蛋白进行偶联,用于制备免疫抗原PEFX-BSA与包被抗原PEFX-OVA,通过紫外扫描和SDS-PAGE对人工抗原进行鉴定,免疫原免疫Balb/c小鼠,获得抗体。试验结果表明,间接ELISA方法检测小鼠血清抗体效价达到1∶6 400。为进一步制备抗PEFX单克隆抗体提供了良好的免疫原。
The carbodimide method was used to couple pefloxacin with carrier protein for the synthesis of immune antigen PEFX-BSA and coating antigen PEFX-OVA.After identification by ultraviolet scanning and SDS-PAGE,the artificial antigen was used to immune Balb/c mice to obtain the antibody.The results showed that the antibody titer in mice serum reached 1∶6 400 by indirect ELISA method.It provided a good immunogen for further preparing the monoclonal antibody of PEFX.
出处
《湖北农业科学》
北大核心
2011年第11期2278-2279,共2页
Hubei Agricultural Sciences
基金
"十一五"国家重大项目(2006BAK02A21)
河南科技学院博士科研启动基金项目(7015)
关键词
培氟沙星
人工抗原
合成
鉴定
pefloxacin
artificial antigens
synthesis
identification
