黄孢原毛平革菌产木素过氧化物酶发酵条件的优化

Optimization of Production of Lignin Peroxidase by Phanerochaete chrysosporium

对白腐菌(Phanerochaete chrysosporium)产木素过氧化物酶的发酵条件进行了研究。在单因素试验的基础上用正交试验进行工艺参数的优化。结果表明,木素过氧化物酶的最佳培养条件为培养13 d、接种0.5 mL,料液比1∶2.5(W/V,g∶mL)、培养温度25℃,酶活为771 U/mL。

The production craft of lignin peroxidase by white-rot fugi Phanerochaete chrysosporium was investigated.On the basis of single factor experiments,orthogonal experiment was used for the optimization of fermentation parameters.The result indicated that the optimal conditions were,the time of culture,13d;the amount of inoculation,0.5 mL;solid to liquid ratio,1∶25（W/V,g∶mL）temperature,25℃;lip activity,771 U/mL.