云南萝芙木中三种生物碱含量的HPLC法测定

Determination of Yohimbine,Ajmalicine and Reserpine in Rauvolfia yunnanensis Tsiang by HPLC

运用高效液相色谱法(HPLC)测定云南萝芙木中育亨宾、阿吗碱、利血平3种生物碱的含量。采用岛津VP-ODS C18色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-(0.004 mol/L二乙胺)水系统,梯度洗脱,流速为1 mL/min,检测波长278 nm,柱温30℃。结果表明,育亨宾在0.140～0.700μg(r=0.999 1)、阿吗碱在0.096～0.480μg(r=0.998 7)、利血平在0.104～0.520μg(r=0.998 8)范围内呈现良好的线性关系,平均加样回收率分别为96.4%、95.5%、96.3%,变异系数分别为1.15%、0.48%、1.14%。该方法可同时检测萝芙木根中育亨宾、阿吗碱、利血平3种生物碱,准确,快速,简便。

A high perfomance liquid chromatographic（HPLC） method was developed for the simultaneous determination of yohimbine,ajmalicine and reserpine in Rauvolfia yunnanensis Tsiang.The analytes were separated on a Shim-pack VP-ODS C18 column（4.6mm×250mm,5μm）and detected at 278nm.The mobile phases were methanol-0.004mol/L 1,2-ethylenediamine for gradient elution at temperature of 30℃.They showed good linearity of the calibration curves for yohimbine,ajmalicine and reserpine in the ranges of 0.140～0.700 μg（r=0,999 1）,0.096～0.480 μg（r=0.998 7）,0.104～0.520 μg（r=0.998 8） respectively.The recoveries were 96.4%,95.5%,96.3% and the relative standard deviations were 1.15%,0.48%,1.14% respectively.The method could be used for the determination of yohimbine,ajmalicine,reserpine in Rauvolfia yunnanensis Tsiang with the advantages of accuracy,speediness and conveniency.