骨关节炎软骨退变与Krüppel样因子-5和基质金属蛋白酶-9表达的关系

Elationship of kriippel-like factor 5 and matrix metalloproteinase-9 expression and neurovascular invasion in osteoarthritis

目的观察Krtippel样因子-5（KLF-5）和基质金属蛋白酶-9（MMP-9）表达活性与人膝关节软骨血管及神经纤维侵入之间的关系。方法从20例行全膝关节置换术的患者中，选取切除的31个关节标本；将每个标本切割成2～3块，并分成磨损组（n=29）和无磨损组（n=17）。结果在磨损组的关节软骨潮线附近有血管化发生，扫描电镜也证实血管结构的存在；关节软骨的神经侵袭仅发生于血管化的标本；与无磨损的关节软骨区比较，磨损区的血管化和神经支配数量明显增加（P〈0．05）。在磨损组的关节软骨中，KLF-5、MMP-9的表达活性明显高丁无磨损组（P〈0．05），而且与血管侵袭程度正相关。结论增加的KLF-5和MMP-9表达可能：是导致骨关节炎软骨退变和血管化的重要机制之一。

Objective To investigate the relationship between the expression of krtippel-like factor （KLF）-5, matrix metalloproteinase-9 （MMP-9） and neurovascular invasion in osteophytes in osteoarthritis （OA）. Methods Thirty-one articular cartilage samples were collected from 20 patients who had undergone total knee arthroplasty （TKA）, and each sample was divided into two groups： no change （NC ,n = 17 ） and severe change （ SC, n = 29） according to Mankin score and safranin O staining. Neurovascular markers protein gone product （PGP） 9. 5 and CD34, and the expression of KLF-5, MMP-9 were detected by using immunohistochemistry. Results Vascular channels were observed in both NC and SC sections. In SC sections, 16/29 （55.2%） sections displayed vessels entering the calcified cartilage, which was more than that in NC group （2/17, 11.8% , P 〈 0. 05 ）. The frequency of neurovascular invasion was significantly different between SC and NC （ P 〈 0. 05 ）. Innervation always accompanied vascular invasion at the osteoehondral junction. The severity of neurovaseular invasion was positively correlated with the expression of KLF-5 and MMP-9 in ehondrocytes at the same site that was significantly different between SC and NC. Conclusion KLF-5-induced MMP-9 expression may be involved in cartilage matrix degradation and vascu- larization.