表阿霉素聚氰基丙烯酸正丁酯磁性纳米粒靶向治疗裸鼠移植性肝癌

Targeting therapy with epirubicin polybutylcyanoacrylate magnetic nanoparticles for transplanted hepatoma in nude mice

目的观察表阿霉素聚氰基丙烯酸正丁酯磁性纳米粒（EPI—PBCA—MNPS）对裸鼠移植性肝癌的作用效果。方法建立Bel-7402裸鼠人肝癌模型，当肿瘤体积长至20～30mm^3时，将其随机分成5组：生理盐水组（NS组）、表阿霉素组（EPI组）、表阿霉素聚氰基丙烯酸正丁酯纳米粒组（EPI—PBCA—NPS组）、表阿霉素聚氰基丙烯酸正丁酯磁性纳米粒组（EPI—PBCA—MNPS组）、表阿霉素聚氰基丙烯酸正丁酯磁性纳米粒组加磁场组（EPI—PBCA—MNPS＋MF组），每组10只，除生理盐水组外，其余各组按照0．002mg／g体质量或相当于40μg/只的表阿霉素静脉注射给药，治疗2周后处死裸鼠，准确测量肿瘤大小并称重，计算瘤体积抑制率及瘤重抑制率，所有肿瘤均编号病理切片，比较治疗前后各组肿瘤细胞坏死的程度。结果治疗后肿瘤体积抑制率、瘤重抑制率除EPI—PBCA—NPS组与EPI—PBCA—MNPS组差异无统计学意义（P〉0．05）外，其余各组间差异有统计学意义（P〈0．05），其中EPI—PBCA—MNP＋MF组（94．26％、80．82％）显著高于其余组，差异有统计学意义（P〈0．01）。病理切片瞳示，EPI—PBCA—MNP＋MF组肿瘤细胞坏死程度最重。结论EPI—PBCA—MNPS具有良好的靶向性，在外加磁场作用下对裸鼠人肝癌模型有显著抑瘤作用。

Objective To observe the effect of epirubicin polybutylcyanoacrylate magnetic nanoparticles （EPI-PBCA-MNPS） on the transplanted hepatoma in nude mice. Methods Human hepatoma cell line Be1-7402 nude mice models were established and divided into 5 groups with each of 10 once gross tumor volmne was up to 20-30 mm3 ：notarial saline group （NS）, epirubicin group （EPI）, epirubicin polybutylcyanoacrylate nanoparticles group （EPI-PBCA-NPS） ,epirubicin polybutylcyanoacrylate magnetic nanoparticles group （EPI-PBCA-MNPS） and epirubicin polybutylcyanoacrylate magnetic nanoparticles ＋ magnetic field group（ EPI-PBCA-MNPS ＋ MF）. Except for NS group, the dosage of epirubicin was 0. 002 mg/g body weight or 40 μg for intravenous injection. After two-weeks therapy, all nude mice models were executed and the tumor volume and weight were measured so as to calculate the volume inhibition ratio and the weight inhibition ratio. Every tumor specimen was made into pathological section and labelled with serial number for comparing neorobiosis degree in different groups before and after therapy. Results There were statistically difference among the other groups （P 〈 0. 05） in the volume inhibition ratio and the weight inhibition ratio after therapy except for EPI-PBCA-NPS and EPI-PBCA-MNPS group （P 〉 0. 05 ）. The two ratios in the EPI-PBCA-MNP ＋ MF group （94. 26% ,80. 82% ） were significantly more than those in other groups （P 〈 0. 01 ）. The pathological sections demonstrated the most severe tumour neorobiosis in the EPI-PBCA-MNP ＋ MF group. Conclusion With the additional magnetic field, EPI-PBCA-MNPS has significant anticancer effect on human hepatoma nude mice models due to its favourable magnetic targeting.