过氧化物酶体增殖物激活受体调控肝癌细胞功能的研究

PPARγ inhibit the cell proliferation and promote the apoptosis of hepatic carcinoma cell

目的构建及鉴定过氧化物酶体增殖物激活受体（PPARγ）过表达肝癌细胞株，观察PPARγ基因对肝癌细胞功能的影响。方法通过构建Ad—PPARγ腺病毒，转染到Hep3B肝癌细胞株，逆转录一聚合酶链反应（RT—PCR）检测基因表达，Western blot对转染后细胞株PPARγ蛋白表达进行检测，并检测Ad—PPARγ／腺病毒转染后Hep3B肝癌细胞的凋亡以及增殖。结果RT-PCR检测发现基因得以成功转染，PPARγ／基因表达增强，CCK-8检测发现转染后72h肝癌细胞增殖能力降低，转染后72h肝癌细胞的凋亡增加13．2％。结论PPARγ基因的表达可抑制肝癌细胞的生长以及促进肝癌细胞的凋亡。

Objective To investigate the peroxisome proliferator-activated receptor （PPAR）γ＇ s function in hepatic carcinoma cell on proliferation and apoptosis. Methods The hepatic carcinoma cell line Hep3B was used to setup a stable overexpression of PPARγ by adenovirus vector. The expression of PPARγ was confirmed by reverse transcription-polymerase chain reaction （RT-PCR） and Western blotting. Cell proliferation and apoptosis of overexpression cell line were measured by CCK-8 kit and flow cytometry assay kit for apoptosis separately. Results The expression vector was successfully transfected into Hep3B cell, and the expression of PPARγ was improved in overexpression Hep3B cell. The cell proliferation was decreased and the apoptosis rate was increased 13.2% in overexpression Hep3B cell after 72 h of transfection. Conclusion PPARγ inhibitor the cell proliferation and promote the apoptosis in hepatic carcinoma cell.