肠道干细胞标记基因Lgr5在结直肠癌中的表达及其意义

Expression and significance of Lgr5 in coiorectal carcinoma

目的探讨结直肠癌组织中Lgr5的表达及其意义。方法采用SP免疫组织化学方法检测139例结直肠癌组织中Lgr5的表达。结果139例结直肠癌中管状腺癌、黏液腺癌和乳头状腺癌的Lgr5蛋白高表达率分别为87．1％、100．0％和75．0％；黏膜及黏膜下层、肌层、浆膜及浆膜外层的lgr5高表达率分别为50．0％、82．6％、93．9％；淋巴结转移阳性组和阴性组的Lgr5高表达率分别为94．6％和81．9％。结直肠癌组织中Lgr5蛋白表达水平与其浸润程度、淋巴结转移、Dukes分期均明显相关（P〈0．05），而与其年龄、性别、组织学类型、病理分期无明显相关（P〉0．05）。结论Lgr5的表达与结直肠癌浸润程度及淋巴结转移状况密切相关，可能参与结直肠癌的发生、发展。

Objective To investigate the expression of leucine-rich-repeat-containing G-proteincoupled receptor 5 （ Lgr5 ） and its significance in colorectal carcinoma. Methods The expression of Lgr5 was examined by immunohistochemistry in 139 cases of colorectal cancer, and its relationship with clinical characteristics was analyzed. Results In 139 colorectal carcinoma samples, the Lgr5 expression rate in tubular adenocarcinoma, mucinous adenocarcinoma, papillary adenocarcinoma was 87. 1% , 100. 0% and 75.0% respectively. The Lgr5 expression rate in mucous layer, muscular layer, and membrane serosa was 50.0% , 82. 6% , 93.9% respectively. High expression levels of Lgr5 were detected in 53 of 56 （94.6%） patients with lymph node metastasis and in 68 of 83 （81.9%） patients without lymph node metastasis. The expression of Lgr5 protein was higher in the tissues with deeper infiltration, advanced Dukes stage, or lymph node or remote metastasis. Conclusion The expression of Lgr5 is significantly correlated with invasion and lymph node metastasis, and may play an important role in the occurrence and development of epithelial colorectal carcinoma. Overexpression of Lgr5 provides an important implication of malignancy and prognosis for colorectal carcinoma.