摘要
采用从汽车修理厂土壤中采集的菌种,借鉴国内外对多环芳烃化合物降解菌的驯化和筛选的方法,在自然条件下驯化、分离、筛选出能降解H205的菌株。研究菌株在不同接种量的条件下以及在不同底物浓度的条件下的降解动力学模型,分析表明,细菌不同接种量时H205的降解为一级反应,接种量为5%、10%、15%、18%、20%时反应式分别为:C=96.75exp(-0.010 6t),C=103.387exp(-0.023t),C=103.234exp(-0.014t),C=102.929exp(-0.011 1t),C=107.429exp(-0.015t)。不同初始底物浓度时H205的降解为零级反应,其降解规律符合Monod方程:1/μ=60.194/sρ+0.443 3。
Bacteria collected from soil in a vehicle repair plant were used with the method in acclimatization and screening of bacteria for PHAs degradation home and abroad..Under natural conditions,strains that can degrade hydroxamic acids collector H205 was cultivated through acclimation,isolation and screening.And this paper studied the effect on this dominant bacteria's biological degradation to induce the best degradation conditions.The kinetic models for degradation in different inoculum and in different substrate concentration were studied.The results show that: Kinetics analysis shows that,H205 degradation reaction with different inoculums follows the first order reaction kinetics equation as follows: C=96.75exp(-0.010 6t),C=103.387exp(-0.023t),C=103.234exp(-0.014t),C=102.929exp(-0.011 1t),C=107.429exp(-0.015t).H205degradation reaction with different initial concentration of substrate follows the zero order enzyme reaction kinetics equation.The biodegradation reaction fits the Monod model as follows: 1/μ=60.194/ρs+0.443 3.
出处
《武汉理工大学学报》
CAS
CSCD
北大核心
2011年第6期103-106,共4页
Journal of Wuhan University of Technology
基金
国家"863"计划(2007AA06Z123)
污染物分析与资源化技术湖北省重点实验室开放基金